Five-year results of a slant-path propagation experiment at 20 GHz in Madrid

Satellite operators are starting to use the Ka-band (30/20 GHz) for communications systems requiring higher traffic capacity. The use of this band is expected to experience a significant growth in the next few years, as several operators have reported plans to launch new satellites with Ka-band capacity. It is worth mentioning the Ka-Sat satellite in Europe, launched in 2010, and ViaSat-1, of 2011, with coverage of USA1. Some other examples can be found in other parts of the World. Recent satellite communications standards, such as DVB-S22 or DVB-RCS3, which provide means to mitigate propagation impairments, have been developed with the objective of improving the use of the Ka-band, in comparison with previous technical standards. In the next years, the ALPHASAT satellite will bring about new opportunities4 for carrying out propagation and telecommunication experiments in the Ka- and Q/V-bands. Commercial uses are focused on the provision of high speed data communications, for Internet access and other applications. In the near future, it is expected that higher and higher data rates will also be needed to broadcast richer multimedia contents, including HD-TV, interactive content or 3D-TV. All of these services may be provided in the future by satellites of the current generation, whose life span can extend up to 2025 in some cases. Depending on local regulations, the available bandwidth for the satellite fixed and broadcasting services in the Ka-band is in excess of several hundred MHz, bidirectional, comprising more than 1 GHz for each sub-band in some cases. In this paper, the results of a propagation experiment that is being carried out at Universidad Politécnica de Madrid (UPM), Spain, are presented5. The objective of the experiment is twofold: gathering experimental time series of attenuation and analyzing them in order to characterize the propagation channel at these frequencies6. The experiment and statistical results correspond to five complete years of measurements. The experiment is described in more detail in Section II. Yearly characteristics of rain attenuation are presented in Section III, whereas Section IV is dedicated to the monthly, seasonal, and hourly characteristics. Section V covers the dynamic characteristics of this propagation effect, just before the conclusions are described in Section VI.

The Pangenome of the Plant-Selected Rhizobium leguminosarum bv viciae Sub-popuations

Rhizobium leguminosarum bv viciae (Rlv) is a bacterium able to establish effective symbioses with four different legume genera: Pisum, Lens, Lathyrus and Vicia. Classic studies using trap plants have previously shown that, given a choice, different plants prefer specific genotypes of rhizobia, which are adapted to the host (1, 2). In previous work we have performed a Pool-Seq analysis bases on pooled DNA samples from Rlv nodule isolates obtained from Pisum sativum, Lens culinaris, Vicia fava and V. sativa plants, used as rhizobial traps. This experiment allowed us to test the host preference hypothesis: different plant hosts select specific sub-populations of rhizobia from the available population present in a given soil. We have observed that plant-selected sub-populations are different at the single nucleotide polymorphism (SNP) level. We have selected individual isolates from each sub-population (9 fava-bean isolates, 14 pea isolates 9 vetch isolates and 9 lentil isolates) and sequenced their genomes at draft level (ca. 30x, 90 contigs). Genomic analyses have been carried out using J-species and CMG-Biotools. All the isolates had similar genome size (7.5 Mb) and number of genes (7,300). The resulting Average Nucleotide Identity (ANIm) tree showed that Rhizobium leguminosarum bv viciae is a highly diverse group. Each plant-selected subpopulation showed a closed pangenome and core genomes of similar size (11,500 and 4,800 genes, respectively). The addition of all four sub-population results in a larger, closed pangenome of 19,040 genes and a core genome of similar size (4,392 genes). Each sub-population contains a characteristic set of genes but no universal, plant-specific genes were found. The core genome obtained from all four sub-populations is probably a representative core genome for Rhizobium leguminosarum, given that the reference genome (Rhizobium leguminosarum bv. viciae strain 3841) contains most of the core genome. We have also analyzed the symbiotic cluster (nod), and different nod cluster genotypes were found in each sub-population. Supported by MINECO (Consolider-Ingenio 2010, MICROGEN Project, CSD2009-00006).